Shimazaki Y, Zhang J, Wakiguchi H, Kurashige T, Sagara Y, Masuoka N, Ohta J, Ubuka T, Kodama H
Department of Pediatrics, Department of Chemistry, Department of Medical Biology, Kochi Medical School, Oko-cho, Kochi, Nankoku, 783-8505, Japan.
Biochem Biophys Res Commun. 1998 Jun 18;247(2):387-91. doi: 10.1006/bbrc.1998.8796.
The priming effect of L-cystathionine sulfoxide, which is one of the unusual cystathionine metabolites found in the urine of patients with cystathioninuria, on the stimulus-induced superoxide generation by human neutrophils was examined. The synthetic L-cystathionine sulfoxide significantly enhanced the superoxide generations induced by N-formyl-methionyl-leucyl-phenylalanine [fMLP], opsonized zymosan [OZ], arachidonic acid [AA], and phorbol 12-myristate 13-acetate [PMA]. Then the synthetic L-cystathionine sulfoxide was separated into two diastereoisomers, CS-I and CS-II, which showed a peak at 76 and 83 min on chromatogram by amino acid analyzer, respectively. CS-I enhanced the superoxide generations induced by AA and PMA but not those induced by fMLP and OZ. On the contrary, CS-II enhanced the superoxide generations induced by fMLP and OZ but not those induced by AA and PMA. The superoxide generation induced by PMA with CS-I was suppressed by H-7 and was enhanced by genistein, while that by fMLP with CS-II was suppressed by genistein and was enhanced by H-7.
