大肠杆菌BE中两个静止孔蛋白基因nmpC和ompN的鉴定与特性分析。
Identification and characterization of two quiescent porin genes, nmpC and ompN, in Escherichia coli BE.
作者信息
Prilipov A, Phale P S, Koebnik R, Widmer C, Rosenbusch J P
机构信息
Department of Microbiology, Biozentrum, University of Basel, Switzerland.
出版信息
J Bacteriol. 1998 Jul;180(13):3388-92. doi: 10.1128/JB.180.13.3388-3392.1998.
Abstract
The genomic DNA of the BE strain of Escherichia coli has been scrutinized to detect porin genes that have not been identified so far. Southern blot analysis yielded two DNA segments which proved highly homologous to, yet distinct from, the ompC, ompF, and phoE porin genes. The two genes were cloned and sequenced. One of them, designated ompN, encodes a porin which, due to low levels of expression, has eluded prior identification. The functional properties (single-channel conductance) of the OmpN porin, purified to homogeneity, closely resemble those of the OmpC porin from E. coli K-12. The second DNA fragment detected corresponds to the nmpC gene, which, due to an insertion of an IS1 element in its coding region, is not expressed in E. coli BE.
摘要
对大肠杆菌BE菌株的基因组DNA进行了仔细检查，以检测迄今尚未鉴定的孔蛋白基因。Southern印迹分析产生了两个DNA片段，它们与ompC、ompF和phoE孔蛋白基因高度同源，但又有所不同。这两个基因被克隆并测序。其中一个命名为ompN，编码一种孔蛋白，由于表达水平低，以前一直未被鉴定出来。纯化至同质的OmpN孔蛋白的功能特性（单通道电导）与大肠杆菌K-12的OmpC孔蛋白非常相似。检测到的第二个DNA片段对应于nmpC基因，由于其编码区域插入了一个IS1元件，该基因在大肠杆菌BE中不表达。
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