Akisaka M, Suzuki M
Okinawa-Asia Research Institute for Medical Science, Faculty of medicine, University of the Ryukyus.
Nihon Ronen Igakkai Zasshi. 1998 Apr;35(4):294-8. doi: 10.3143/geriatrics.35.294.
We analyzed HLA class II genes of Okinawan centenarians using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to identify primary genetic factors in the major histo-compatibility complex (MHC) region associated with human longevity. Gene frequencies of centenarians were compared with those of normal adults of the same ethnicity who were selected in the same vicinity as the centenarians. The following differences were identified in the HLA-DQB1 and DQA1 genes: the frequencies of DQB10503, DQA10101 (04) and DQA105 were increased in the centenarians, whereas those of DQA10102, DQA10103 and DQB10604 were decreased. Similarly, for the DRB1 gene, the frequencies of DRB10101, DRB11201 and DRB11401 were increased in the centenarians, whereas those of DRB10403 and DRB1*1302 were decreased. These data suggest that several alleles of the HLA-DRB1 and/or HLA-DQ genes are involved in human longevity.
我们采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析了冲绳百岁老人的HLA II类基因，以确定主要组织相容性复合体（MHC）区域中与人类长寿相关的主要遗传因素。将百岁老人的基因频率与在与百岁老人相同地点选取的同种族正常成年人的基因频率进行比较。在HLA-DQB1和DQA1基因中发现了以下差异：百岁老人中DQB10503、DQA10101（04）和DQA105的频率增加，而DQA10102、DQA10103和DQB10604的频率降低。同样，对于DRB1基因，百岁老人中DRB10101、DRB11201和DRB11401的频率增加，而DRB10403和DRB1*1302的频率降低。这些数据表明，HLA-DRB1和/或HLA-DQ基因的几个等位基因与人类长寿有关。
