Gough K H, Shukla D D, Pallaghy C K
School of Botany, La Trobe University, Bundoora, Victoria, Australia.
Arch Virol. 1998;143(5):1015-20. doi: 10.1007/s007050050350.
The morphology of an Australian strain of Johnsongrass mosaic potyvirus (JGMV Krish-infecting strain), capable of infecting sorghums carrying the Krish potyvirus resistance gene, was investigated and the base sequence of the coat protein region determined. Under the electron microscope the virus was indistinguishable from the wild type prevalent in Australia, JGMV-Jg. However, there were some significant changes in the inferred amino acid sequence in both the N-terminus and the core regions of the coat protein. Some of these amino acid changes may be responsible for breaking the resistance of sorghums carrying the Krish virus resistance gene. In the discussion mention is made of a preliminary result with a mutated in vitro transcript which supports this suggestion.
对一种能够感染携带克里什马铃薯Y病毒抗性基因高粱的澳大利亚约翰逊草花叶马铃薯Y病毒（JGMV克里什感染株）的形态进行了研究，并测定了外壳蛋白区域的碱基序列。在电子显微镜下，该病毒与澳大利亚流行的野生型JGMV-Jg无法区分。然而，外壳蛋白的N端和核心区域的推断氨基酸序列有一些显著变化。其中一些氨基酸变化可能是导致携带克里什病毒抗性基因的高粱抗性被打破的原因。在讨论中提到了一个关于体外转录突变体的初步结果，该结果支持了这一观点。
