Dimier I H, Quéré P, Naciri M, Bout D T
CJF INSERM 93-09 Immunologie des Maladies Infectieuses, Equipe associée INRA Immunologie Parasitaire UFR des Sciences Pharmaceutiques, Tours, France.
Avian Dis. 1998 Apr-Jun;42(2):239-47.
Pretreatment of chicken macrophages or fibroblasts with supernatants from concanavalin A-stimulated spleen cells or from the virus-transformed cell line reticuloendotheliovirus as source of interferon gamma (IFN-gamma) slows down subsequent sporozoite replication in the cells. To identify the presence of IFN-gamma, we combined four typical activities of IFN-gamma: inhibition of cytopathic effect of vesicular stomatitis virus on IFN-gamma-treated fibroblasts, cytostatic activity of IFN-gamma-activated macrophages, induction of major histocompatibility complex II antigen expression on IFN-gamma-activated fibroblasts and macrophages, and induction of nitrite production in macrophages. We have shown that chicken fibroblasts and macrophages possess a microbiostatic capacity once they are able to prevent the otherwise unchecked intracellular replication of Eimeria tenella following activation with culture supernatants identified as containing a strong IFN-gamma activity.
用来自伴刀豆球蛋白A刺激的脾细胞的上清液或来自病毒转化细胞系网状内皮病毒（作为γ干扰素（IFN-γ）的来源）对鸡巨噬细胞或成纤维细胞进行预处理，会减缓随后子孢子在细胞中的复制。为了鉴定IFN-γ的存在，我们结合了IFN-γ的四种典型活性：抑制水疱性口炎病毒对IFN-γ处理的成纤维细胞的细胞病变效应、IFN-γ激活的巨噬细胞的细胞生长抑制活性、IFN-γ激活的成纤维细胞和巨噬细胞上主要组织相容性复合体II抗原表达的诱导以及巨噬细胞中亚硝酸盐产生的诱导。我们已经表明，鸡成纤维细胞和巨噬细胞一旦能够在用鉴定为含有强IFN-γ活性的培养上清液激活后防止柔嫩艾美耳球虫不受控制的细胞内复制，就具有微生物抑制能力。
