Sørensen K J, Strandbygaard B, Bøtner A, Madsen E S, Nielsen J, Have P
Danish Veterinary Institute for Virus Research, Kalvehave, Denmark.
Vet Microbiol. 1998 Feb 28;60(2-4):169-77. doi: 10.1016/s0378-1135(98)00159-x.
A double blocking ELISA was developed in order to satisfy the need for large scale serological screening for PRRS and simultaneous distinction between infection with European and American strains of PRRSV in pig herds. The Immunoperoxidase monolayer assay (IPMA) and the double blocking ELISA enabled distinction on serological basis between infection with European and American strains of PRRSV. The distinction was possible from about day 7 after infection of pigs with PRRSV. The double blocking ELISA enabled the distinction at later stages of infection compared to the IPMA, irrespective of the strain involved.
为满足猪群中对猪繁殖与呼吸综合征（PRRS）进行大规模血清学筛查以及同时区分欧洲株和美洲株PRRS病毒感染的需求，开发了一种双阻断ELISA方法。免疫过氧化物酶单层试验（IPMA）和双阻断ELISA能够在血清学基础上区分欧洲株和美洲株PRRS病毒的感染。自猪感染PRRS病毒后约第7天起即可进行区分。与IPMA相比，双阻断ELISA能够在感染后期进行区分，且与所涉及的毒株无关。
