Biri H, Oztürk H S, Büyükkoçak S, Kaçmaz M, Cimen M Y, Unal D, Birey M, Bozkirli I, Durak I
Department of Urology, Gazi University Medical Faculty, Ankara, Turkey.
Nephron. 1998;79(2):181-5. doi: 10.1159/000045022.
Antioxidant defense potential, malondialdehyde (MDA) levels, and relative hydroxyl radical (OH.) concentrations were measured in order to establish the effects of extracorporeal shock wave lithotripsy (ESWL) on free radical production and antioxidant defense potential of the rabbit kidney tissues. Electron microscopic examination was also performed to observe ultrastructural changes. The antioxidant defense potential of the ESWL-treated tissues was found to be reduced, and the MDA levels increased as compared with controls. Vitamin (vitamin E plus C combination) pretreatment ameliorated antioxidant defense potential in part, prevented increases in MDA levels in the ESWL-treated tissues, and increased the antioxidant defense potential in the control kidney tissues. After ESWL, a significant amount of OH. radical was measured in the affected tissue. This revealed the source of oxidant stress and peroxidation reactions in the ESWL-treated kidney tissue. Vitamin pretreatment caused significant reduction in the OH. radical concentration. In the electron microscopic investigation, some significant subcellular changes, such as endothelial injury, loss of foot processes, damage of glomerular basal membrane, etc., were observed in the ESWL-treated renal tissue slices. Vitamin pretreatment to a great extent prevented formation of these subcellular changes. Our results suggest that the antioxidant capacity of the kidney tissue was reduced after ESWL treatment and that the tissue was exposed to oxidant stress. Vitamin pretreatment exerted significant protection against the radical damage.
为了确定体外冲击波碎石术（ESWL）对兔肾组织自由基产生和抗氧化防御潜能的影响，对其抗氧化防御潜能、丙二醛（MDA）水平和相对羟自由基（OH·）浓度进行了测量。还进行了电子显微镜检查以观察超微结构变化。结果发现，与对照组相比，接受ESWL治疗的组织的抗氧化防御潜能降低，MDA水平升高。维生素（维生素E加C联合使用）预处理部分改善了抗氧化防御潜能，防止了ESWL治疗组织中MDA水平的升高，并提高了对照肾组织的抗氧化防御潜能。ESWL治疗后，在受影响的组织中检测到大量的OH·自由基。这揭示了ESWL治疗的肾组织中氧化应激和过氧化反应的来源。维生素预处理使OH·自由基浓度显著降低。在电子显微镜检查中，在ESWL治疗的肾组织切片中观察到一些明显的亚细胞变化，如内皮损伤、足突消失、肾小球基底膜损伤等。维生素预处理在很大程度上防止了这些亚细胞变化的形成。我们的结果表明，ESWL治疗后肾组织的抗氧化能力降低，组织受到氧化应激。维生素预处理对自由基损伤具有显著的保护作用。
