He Q, Khanna P, Srivastava S, van Kuijk F J, Ansari N H
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555-0647, USA.
Biochem Biophys Res Commun. 1998 Jun 29;247(3):719-22. doi: 10.1006/bbrc.1998.8845.
Aldose reductase has been purified to homogeneity from bovine retina. It has an apparent molecular weight of 32,000 daltons and shares immunological and kinetic properties with the much studied aldose reductases purified from various sources. Retinal aldose reductase displays a K(m) of approximately 40 microM with 4-hydroxynonenal and 4-hydroxyhexenal, the oxidation end products of arachidonic and docosahexanoeic acids, respectively. It therefore appears that aldose reductase may constitute a major detoxification route of these toxic aldehydes in the retina.
醛糖还原酶已从牛视网膜中纯化至同质。它的表观分子量为32,000道尔顿，与从各种来源纯化的、已被广泛研究的醛糖还原酶具有免疫和动力学特性。视网膜醛糖还原酶对4-羟基壬烯醛和4-羟基己烯醛的米氏常数（K(m)）约为40微摩尔，它们分别是花生四烯酸和二十二碳六烯酸的氧化终产物。因此，醛糖还原酶似乎可能构成视网膜中这些有毒醛类的主要解毒途径。
