Srivastava S, Chandra A, Bhatnagar A, Srivastava S K, Ansari N H
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555-0647, USA.
Biochem Biophys Res Commun. 1995 Dec 26;217(3):741-6. doi: 10.1006/bbrc.1995.2835.
There is increasing evidence that lipid aldehydes generated endogenously during the process of degradation of lipid peroxides, are causally involved in the pathophysiology effects associated with oxidative stress. We report here that 4-hydroxynonenal (HNE), which is one of the most abundant and toxic lipid aldehyde can be efficiently detoxified by the aldo-keto reductase, aldose reductase, purified from bovine lens. The enzyme displays a Km of congruent to 9 microM for HNE and 34 microM for the glutathione adduct of HNE (HNE-GS) assigning HNE and HNE-GS to be the best natural substrates of aldose reductase known so far and exposing a new efficient detoxification route of HNE.
越来越多的证据表明，脂质过氧化物降解过程中内源性产生的脂质醛与氧化应激相关的病理生理效应存在因果关系。我们在此报告，4-羟基壬烯醛（HNE）是最丰富且毒性最强的脂质醛之一，它可以被从牛晶状体中纯化得到的醛糖还原酶（aldo-keto reductase）有效解毒。该酶对HNE的Km值约为9微摩尔，对HNE的谷胱甘肽加合物（HNE-GS）的Km值为34微摩尔，这表明HNE和HNE-GS是迄今为止已知的醛糖还原酶的最佳天然底物，并揭示了一条新的HNE高效解毒途径。
