Kimura M, Shingu Y, Yoneyama K, Yamaguchi I
Microbial Toxicology Laboratory, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Biosci Biotechnol Biochem. 1998 May;62(5):1033-6. doi: 10.1271/bbb.62.1033.
A structural gene of Tri101, which encodes trichothecene 3-O-acetyltransferase, was isolated as a 3-kb XhoI-XbaI fragment from the trichothecene producer Fusarium graminearum strain F15. The gene contained no introns, and the coding region was 0.7-kb downstream of a putative UTP-ammonia ligase gene which obviously is not related to the biosynthesis of trichothecenes. Tri101 was expressed when T-2 toxin was added, but this induction was not dependent on the expression level of Tri6, a transcription activator gene in the trichothecene biosynthetic and regulatory gene cluster.
从产单端孢霉烯族毒素的禾谷镰刀菌菌株F15中分离出Tri101的一个结构基因，该基因编码单端孢霉烯3 - O - 乙酰转移酶，它是一个3 kb的XhoI - XbaI片段。该基因不含内含子，其编码区位于一个假定的UTP - 氨连接酶基因下游0.7 kb处，该假定基因显然与单端孢霉烯族毒素的生物合成无关。添加T - 2毒素时Tri101会表达，但这种诱导不依赖于Tri6（单端孢霉烯族毒素生物合成和调控基因簇中的一个转录激活基因）的表达水平。
