禾谷镰刀菌中编码单端孢霉烯3 - O - 乙酰转移酶的基因TRI101的破坏。
Disruption of TRI101, the gene encoding trichothecene 3-O-acetyltransferase, from Fusarium sporotrichioides.
作者信息
McCormick S P, Alexander N J, Trapp S E, Hohn T M
机构信息
Mycotoxin Research Unit, USDA/ARS National Center for Agricultural Utilization Research, Peoria, Illinois 61604, USA.
出版信息
Appl Environ Microbiol. 1999 Dec;65(12):5252-6. doi: 10.1128/AEM.65.12.5252-5256.1999.
Abstract
We screened a Fusarium sporotrichioides NRRL 3299 cDNA expression library in a toxin-sensitive Saccharomyces cerevisiae strain lacking a functional PDR5 gene. Fourteen yeast transformants were identified as resistant to the trichothecene 4,15-diacetoxyscirpenol, and each carried a cDNA encoding the trichothecene 3-O-acetyltransferase that is the F. sporotrichioides homolog of the Fusarium graminearum TRI101 gene. Mutants of F. sporotrichioides NRRL 3299 produced by disruption of TRI101 were altered in their abilities to synthesize T-2 toxin and accumulated isotrichodermol and small amounts of 3, 15-didecalonectrin and 3-decalonectrin, trichothecenes that are not observed in cultures of the parent strain. Our results indicate that TRI101 converts isotrichodermol to isotrichodermin and is required for the biosynthesis of T-2 toxin.
摘要
我们在缺乏功能性PDR5基因的毒素敏感型酿酒酵母菌株中筛选了禾谷镰刀菌NRRL 3299的cDNA表达文库。鉴定出14个对单端孢霉烯族毒素4,15 - 二乙酰氧基雪腐镰刀菌烯醇具有抗性的酵母转化体，每个转化体都携带一个编码单端孢霉烯族毒素3 - O - 乙酰转移酶的cDNA，该酶是禾谷镰刀菌TRI101基因的禾谷镰刀菌同源物。通过破坏TRI101产生的禾谷镰刀菌NRRL 3299突变体在合成T - 2毒素的能力上发生了改变，并积累了异毛壳菌素以及少量的3,15 - 二去甲雪腐镰刀菌烯醇和3 - 去甲雪腐镰刀菌烯醇，这些单端孢霉烯族毒素在亲本菌株的培养物中未观察到。我们的结果表明，TRI101将异毛壳菌素转化为异毛壳菌毒素，并且是T - 2毒素生物合成所必需的。
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