检测用细胞因子肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）刺激的人内皮细胞上的Fcγ受体。
Detection of Fcgamma receptors on human endothelial cells stimulated with cytokines tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma).
作者信息
Pan L F, Kreisle R A, Shi Y D
机构信息
Department of Biophysics, Shanghai Medical University, People's Republic of China.
出版信息
Clin Exp Immunol. 1998 Jun;112(3):533-8. doi: 10.1046/j.1365-2249.1998.00597.x.
This investigation was conducted to detect Fcgamma receptors (FcgammaR) on cytokine-stimulated human endothelial cells (EC) by measuring anti-FcgammaR MoAb binding with an ELISA. TNF-alpha and IFN-gamma significantly increased the expression of FcgammaR type II (FcgammaRII) and type III (FcgammaRIII) on aortic EC. Simultaneous treatment with both cytokines had a synergistic effect and pretreatment of EC with IFN-gamma augmented the effect of TNF-alpha. The greatest effect was the increase (up to four-to-six-fold) in expression of FcgammaRII found by the simultaneous treatment of aortic EC with both cytokines. The receptors were expressed on the cell surface and showed receptor capping after incubation at 37 degrees C. This study showed that the inflammatory cytokines TNF-alpha and IFN-gamma enhanced low-affinity FcgammaR expression on human EC in vitro. The expression of FcgammaR may contribute to the specific localization of circulating immune complexes on blood vessels in areas of vasculitis.
本研究旨在通过酶联免疫吸附测定法（ELISA）检测抗Fcγ受体单克隆抗体（MoAb）结合情况，以检测细胞因子刺激的人内皮细胞（EC）上的Fcγ受体（FcγR）。肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）显著增加主动脉内皮细胞上II型Fcγ受体（FcγRII）和III型Fcγ受体（FcγRIII）的表达。两种细胞因子同时处理具有协同作用，并且用IFN-γ预处理内皮细胞可增强TNF-α的作用。最大的效应是两种细胞因子同时处理主动脉内皮细胞时发现FcγRII表达增加（高达四至六倍）。这些受体表达于细胞表面，在37℃孵育后出现受体聚集。本研究表明，炎性细胞因子TNF-α和IFN-γ在体外增强人内皮细胞上低亲和力FcγR的表达。FcγR的表达可能有助于循环免疫复合物在血管炎区域的血管上特异性定位。
Zotero 插件