Stucchi A F, Nicolosi R J, Karge W H, Ausman L M, Ordovas J M
Center for Cardiovascular Disease Control, Department of Health and Clinical Sciences, University of Massachusetts-Lowell, Lowell, MA 01854, USA.
J Nutr. 1998 Jul;128(7):1104-13. doi: 10.1093/jn/128.7.1104.
To examine the mechanism(s) underlying the cholesterolemic response to dietary cholesterol and saturated fatty acids, low density lipoprotein (LDL) metabolism was studied in two groups of cynomolgus monkeys fed diets containing 30 or 36% of total energy as fat. At each dietary fat level, the same group of monkeys was sequentially fed three dietary cholesterol concentrations as egg yolk in the following sequence: low (0.01 mg/kJ), medium (0.03 mg/kJ) and high (0.05 mg/kJ) for 30, 32 and 24 wk, respectively. Dietary polyunsaturated and monounsaturated fatty acids were the same in the two groups; the 6% difference in fat was due to the saturated fatty acids, 12:0 and 14:0. Serum total cholesterol, LDL cholesterol and LDL apolipoprotein B concentrations increased (P < 0.05) with dietary cholesterol in a dose-dependent manner in both fat groups. These elevations were the result of generally increasing LDL apolipoprotein B production rates, concomitant with reduced LDL apolipoprotein B fractional clearance at the high cholesterol intake. Serum HDL cholesterol and HDL apolipoprotein A-I concentrations were not affected in a consistent manner. These results demonstrate that cynomolgus monkeys are hyperresponsive to dietary cholesterol compared with humans, suggesting that this model may be useful in identifying metabolic and genetic predictors for hyperresponsiveness to dietary cholesterol in humans as well as assessing the metabolic heterogeneity of responses to dietary cholesterol.
为研究饮食中胆固醇和饱和脂肪酸引起胆固醇血症反应的潜在机制，对两组食蟹猴的低密度脂蛋白（LDL）代谢进行了研究，这两组猴子的饮食中脂肪提供的能量占总能量的30%或36%。在每个饮食脂肪水平下，同一组猴子按以下顺序依次喂食三种不同胆固醇浓度的蛋黄饮食：低（0.01 mg/kJ）、中（0.03 mg/kJ）和高（0.05 mg/kJ），分别持续30、32和24周。两组饮食中的多不饱和脂肪酸和单不饱和脂肪酸相同；脂肪含量6%的差异是由于饱和脂肪酸12:0和14:0造成的。在两个脂肪组中，血清总胆固醇、LDL胆固醇和LDL载脂蛋白B浓度均随饮食胆固醇的增加呈剂量依赖性升高（P<0.05）。这些升高是由于LDL载脂蛋白B生成率普遍增加，同时在高胆固醇摄入量时LDL载脂蛋白B的部分清除率降低所致。血清HDL胆固醇和HDL载脂蛋白A-I浓度没有受到一致的影响。这些结果表明，与人类相比，食蟹猴对饮食胆固醇反应过度，这表明该模型可能有助于识别人类对饮食胆固醇反应过度的代谢和遗传预测指标，以及评估对饮食胆固醇反应的代谢异质性。
