[HPLC analysis of nonsteroidal antirheumatic agents in biological materials. III. Pyrazolidinediones].

The present paper surveys published HPLC methods estimating pyrazolidinediones in biological materials and an HPLC method for estimation of kebuzone in the samples of whole blood, isolated erythrocytes and plasma. An addition of propyl gallate prevented undesired oxidation of kebuzone. For liquid-liquid extraction, kebuzone was analysed on the reverse phases Separon SGX C-18 with the mobile phase methanol--water (pH 2.7) and detected at 247 nm. The elaborated method was used in a pharmacokinetic study of kebuzone on rabbits.