Nishiyori A, Minami M, Ohtani Y, Takami S, Yamamoto J, Kawaguchi N, Kume T, Akaike A, Satoh M
Department of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
FEBS Lett. 1998 Jun 12;429(2):167-72. doi: 10.1016/s0014-5793(98)00583-3.
Localization of the mRNAs for fractalkine, a CX3C chemokine, and for its receptor CX3CR1 was investigated in the rat brain. In situ hybridization study revealed that fractalkine mRNA was dominantly expressed in neuronal cells particularly in the olfactory bulb, cerebral cortex, hippocampus, caudate putamen and nucleus accumbens. In vitro study using enriched neuronal or glial culture supported the dominant expression of fractalkine mRNA in neurons. On the other hand, CX3CR1 mRNA was dominantly expressed in glial cells throughout the whole brain. The in vitro study suggested the cells expressing CX3CR1 mRNA are microglia, not astrocytes or neurons. Fractalkine appears to function as a signal molecule from neuron to microglia.
在大鼠脑中研究了CX3C趋化因子fractalkine及其受体CX3CR1的mRNA定位。原位杂交研究显示，fractalkine mRNA主要在神经元细胞中表达，特别是在嗅球、大脑皮层、海马体、尾状壳核和伏隔核中。使用富集神经元或神经胶质细胞培养的体外研究支持了fractalkine mRNA在神经元中的主要表达。另一方面，CX3CR1 mRNA在全脑的神经胶质细胞中主要表达。体外研究表明，表达CX3CR1 mRNA的细胞是小胶质细胞，而非星形胶质细胞或神经元。Fractalkine似乎作为一种从神经元到小胶质细胞的信号分子发挥作用。
