从皮炎芽生菌酵母相细胞壁中分离纯化皮肤试验抗原
Isolation of a purified skin test antigen from Blastomyces dermatitidis yeast-phase cell wall.
作者信息
Lancaster M V, Sprouse R F
出版信息
Infect Immun. 1976 Sep;14(3):623-5. doi: 10.1128/iai.14.3.623-625.1976.
Abstract
Preparative polyacrylamide gel electrophoresis was used to isolate individual components of an alkaline-soluble-water-soluble fraction of the cell wall of Blastomyces dermatitidis yeast phase. One component isolated demonstrated exceptional specificity and reactivity when tested on guinea pigs infected with B. dermatitidis. This component displayed no cross-reactivity when tested on animals infected with Histoplasma capsulatum. The significance of isolation of a purified, specific antigen is discussed.
摘要
制备性聚丙烯酰胺凝胶电泳用于分离皮炎芽生菌酵母相细胞壁碱溶性-水溶性组分中的各个成分。分离出的一种成分在感染皮炎芽生菌的豚鼠身上进行测试时表现出非凡的特异性和反应性。在感染荚膜组织胞浆菌的动物身上进行测试时，该成分没有交叉反应性。本文讨论了分离纯化特异性抗原的意义。
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DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
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