从放线菌素纯化衍生物中对皮肤试验抗原进行制备性等速电泳分离。
Preparative isotachophoretic separation of skin test antigens from blastomycin purified derivative.
作者信息
Lancaster M V, Sprouse R F
出版信息
Infect Immun. 1976 Mar;13(3):758-62. doi: 10.1128/iai.13.3.758-762.1976.
Abstract
This investigation examined the theoretical and practical parameters requisite to preparatory istachophoretic separation of blastomycin purified derivative. It resulted in a relatively simple, two-step procedure for preparation of blastomycin antigens in milligram quantitites that exhibited sensitivity and specificity in experimentally infected guinea pigs. Analysis of the nine isotachophoretic fractions for skin test sensitivity and specificity provided some insight into the generally accepted unreliability of blastomycin when used for immunological evaluation.
摘要
本研究考察了制备布拉氏菌素纯化衍生物等速电泳分离所需的理论和实际参数。研究得出了一个相对简单的两步法，可制备毫克量的布拉氏菌素抗原，这些抗原在实验感染的豚鼠中表现出敏感性和特异性。对九个等速电泳级分进行皮肤试验敏感性和特异性分析，为布拉氏菌素用于免疫评估时普遍公认的不可靠性提供了一些见解。
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