从放线菌素纯化衍生物中对皮肤试验抗原进行制备性等速电泳分离。
Preparative isotachophoretic separation of skin test antigens from blastomycin purified derivative.
作者信息
Lancaster M V, Sprouse R F
出版信息
Infect Immun. 1976 Mar;13(3):758-62. doi: 10.1128/iai.13.3.758-762.1976.
Abstract
This investigation examined the theoretical and practical parameters requisite to preparatory istachophoretic separation of blastomycin purified derivative. It resulted in a relatively simple, two-step procedure for preparation of blastomycin antigens in milligram quantitites that exhibited sensitivity and specificity in experimentally infected guinea pigs. Analysis of the nine isotachophoretic fractions for skin test sensitivity and specificity provided some insight into the generally accepted unreliability of blastomycin when used for immunological evaluation.
摘要
本研究考察了制备布拉氏菌素纯化衍生物等速电泳分离所需的理论和实际参数。研究得出了一个相对简单的两步法，可制备毫克量的布拉氏菌素抗原，这些抗原在实验感染的豚鼠中表现出敏感性和特异性。对九个等速电泳级分进行皮肤试验敏感性和特异性分析，为布拉氏菌素用于免疫评估时普遍公认的不可靠性提供了一些见解。
相似文献
1
Preparative isotachophoretic separation of skin test antigens from blastomycin purified derivative.
Infect Immun. 1976 Mar;13(3):758-62. doi: 10.1128/iai.13.3.758-762.1976.
2
Isolation of a purified skin test antigen from Blastomyces dermatitidis yeast-phase cell wall.
Infect Immun. 1976 Sep;14(3):623-5. doi: 10.1128/iai.14.3.623-625.1976.
3
In vivo and in vitro cell-mediated immune responses to a cell wall antigen of Blastomyces dermatitidis.
Infect Immun. 1977 Feb;15(2):429-35. doi: 10.1128/iai.15.2.429-435.1977.
4
Isolation of skin test-active preparations from yeast-phase cells of Blastomyces dermatitidis.
Infect Immun. 1974 Jul;10(1):42-7. doi: 10.1128/iai.10.1.42-47.1974.
5
Isoelectric focusing and ELISA evaluation of a Blastomyces dermatitidis human isolate.
Mycopathologia. 2007 Aug;164(2):73-6. doi: 10.1007/s11046-007-9033-8. Epub 2007 Jun 20.
6
Use of an alkali-soluble water-soluble extract of Blastomyces dermatitidis yeast-phase cell walls and isoelectrically focused components in peripheral lymphocyte transformations.
Infect Immun. 1978 Feb;19(2):411-5. doi: 10.1128/iai.19.2.411-415.1978.
7
Distribution of Blastomyces dermatitidis in dogs with skin test and serologic results following airborne infection.
Sabouraudia. 1975 Jul;13(2):192-9.
8
Laboratory procedures for potency testing of blastomycin, histoplasmin, and coccidioidin.
Proc Soc Exp Biol Med. 1972 Jun;140(2):556-9. doi: 10.3181/00379727-140-36502.
9
Comparative studies on the detection of delayed dermal hypersensitivity in experimental animals with lysate and filtrate antigens of Blastomyces dermatitidis.
Mycoses. 1994 Jun-Jul;37(5-6):149-53. doi: 10.1111/j.1439-0507.1994.tb00292.x.
10
Antigens of alternaria. I. Isolation and partial characterization of a basic peptide allergen.
J Allergy Clin Immunol. 1983 Mar;71(3):277-82. doi: 10.1016/0091-6749(83)90081-7.
引用本文的文献
1
Antigens and chemical composition of Blastomyces dermatitidis.
Mycopathologia. 1982 Apr 23;78(1):47-63. doi: 10.1007/BF00436581.
2
Immunohistopathological studies of blastomycosis: the use of labeled specific antigens in a hamster model of disease.
Mycopathologia. 1984 Mar 15;85(1-2):17-20. doi: 10.1007/BF00436697.
3
Isolation of a purified skin test antigen from Blastomyces dermatitidis yeast-phase cell wall.
Infect Immun. 1976 Sep;14(3):623-5. doi: 10.1128/iai.14.3.623-625.1976.
4
Use of an alkali-soluble water-soluble extract of Blastomyces dermatitidis yeast-phase cell walls and isoelectrically focused components in peripheral lymphocyte transformations.
Infect Immun. 1978 Feb;19(2):411-5. doi: 10.1128/iai.19.2.411-415.1978.
5
Cross-reactivity between antigens of Coccidioides immitis, Histoplasma capsulatum and Blastomyces dermatitidis in lymphocyte transformation assays.
Infect Immun. 1979 Sep;25(3):932-8. doi: 10.1128/iai.25.3.932-938.1979.
6
In vivo and in vitro cell-mediated immune responses to a cell wall antigen of Blastomyces dermatitidis.
Infect Immun. 1977 Feb;15(2):429-35. doi: 10.1128/iai.15.2.429-435.1977.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
CLINICAL AND EPIDEMIOLOGIC STUDIES ON SEVENTY-NINE CANINE BLASTOMYCOSIS CASES IN ARKANSAS.
Am J Epidemiol. 1965 Mar;81:164-79. doi: 10.1093/oxfordjournals.aje.a120505.
3
Micro isoelectric focusing in polyacrylamide gel columns.
Anal Biochem. 1968 Dec;26(3):480-2. doi: 10.1016/0003-2697(68)90219-4.
4
Histoplasmin potency as affected by culture age.
Sabouraudia. 1968 Oct;6(4):273-84. doi: 10.1080/00362176885190551.
5
Prevalence and incidence studies of human and canine blastomycosis. 1. Cases in the United States, 1885-1968.
Am Rev Respir Dis. 1970 Jul;102(1):60-7. doi: 10.1164/arrd.1970.102.1.60.
6
Yeast- and mycelial-phase antigens of Blastomyces dermatitidis: comparison using disc gel electrophoresis.
Infect Immun. 1974 Jul;10(1):48-53. doi: 10.1128/iai.10.1.48-53.1974.
7
General aspects of analytical isotachophoresis of proteins in polyacrylamide gels.
Anal Biochem. 1972 Jan;45(1):192-201. doi: 10.1016/0003-2697(72)90019-x.
Zotero 插件