Reubel G H, Kimsey R B, Barlough J E, Madigan J E
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616, USA.
J Clin Microbiol. 1998 Jul;36(7):2131-4. doi: 10.1128/JCM.36.7.2131-2134.1998.
We report the experimental transmission of Ehrlichia equi from naturally infected Ixodes pacificus ticks to horses. Three weeks after exposure to ticks, two of three horses developed clinical signs compatible with E. equi infection, while one horse remained asymptomatic. 16S rRNA gene PCR of blood leukocyte lysates was positive for all horses at various time points; two horses seroconverted. The 16S rRNA gene sequences amplified from tick-exposed horses showed more than 99% homology to corresponding fragments of the 16S rRNA genes of E. equi, Ehrlichia phagocytophila, and the human granulocytic ehrlichiosis agent.
我们报告了马埃里希体（Ehrlichia equi）从自然感染的太平洋硬蜱（Ixodes pacificus）向马的实验性传播情况。在接触蜱虫三周后，三匹马中有两匹出现了与马埃里希体感染相符的临床症状，而一匹马仍无症状。在不同时间点，所有马的血液白细胞裂解物的16S rRNA基因PCR检测均呈阳性；两匹马血清学转换。从接触蜱虫的马中扩增出的16S rRNA基因序列与马埃里希体、嗜吞噬细胞埃里希体及人类粒细胞埃里希体病原体的16S rRNA基因相应片段显示出超过99%的同源性。
