Chistoserdova L, Vorholt J A, Thauer R K, Lidstrom M E
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
Science. 1998 Jul 3;281(5373):99-102. doi: 10.1126/science.281.5373.99.
Methanogenic and sulfate-reducing Archaea are considered to have an energy metabolism involving C1 transfer coenzymes and enzymes unique for this group of strictly anaerobic microorganisms. An aerobic methylotrophic bacterium, Methylobacterium extorquens AM1, was found to contain a cluster of genes that are predicted to encode some of these enzymes and was shown to contain two of the enzyme activities and one of the methanogenic coenzymes. Insertion mutants were all unable to grow on C1 compounds, suggesting that the archaeal enzymes function in aerobic C1 metabolism. Thus, methylotrophy and methanogenesis involve common genes that cross the bacterial/archaeal boundaries.
产甲烷古菌和硫酸盐还原古菌被认为具有涉及C1转移辅酶和该组严格厌氧微生物特有的酶的能量代谢。一种需氧甲基营养细菌，即扭脱甲基杆菌AM1，被发现含有一组预计编码其中一些酶的基因，并被证明含有两种酶活性和一种产甲烷辅酶。插入突变体均无法在C1化合物上生长，这表明古菌酶在需氧C1代谢中起作用。因此，甲基营养和产甲烷作用涉及跨越细菌/古菌界限的共同基因。
