Dixit B L, Gupta C M
Division of Membrane Biology, Central Drug Research Institute, Lucknow, India.
Eur J Biochem. 1998 May 15;254(1):202-6. doi: 10.1046/j.1432-1327.1998.2540202.x.
Transbilayer phosphatidylethanolamine (PtdEtn) movements in the plasma membrane of Saccharomyces cerevisiae are regulated by an ATP-dependent, protein-mediated process(es). To examine whether this process is influenced by the actin cytoskeleton, we have studied the PtdEtn translocation in S. cerevisiae cells after treatment with microfilament disrupting and microtubule-disrupting agents. PtdEtn translocation was studied by measuring the external PtdEtn levels, using fluorescamine as the external membrane probe, in the ATP-depleted, ATP-depleted and repleted, and N-ethylmaleimide-treated cells. The microfilaments and microtubules were disrupted by treatment with various cytochalasins and colchicine (or benomyl) respectively PtdEtn translocation became abnormal in the cytochalasin-treated cells but not in cells that were treated with microtubule-disrupting agents, such as colchicine or benomyl. These results have been interpreted to suggest that the actin cytoskeleton is involved in regulating the PtdEtn translocase activity in the yeast cell plasma membrane.
酿酒酵母质膜中的跨膜磷脂酰乙醇胺（PtdEtn）运动受ATP依赖的蛋白质介导过程调控。为了研究该过程是否受肌动蛋白细胞骨架的影响，我们在用微丝破坏剂和微管破坏剂处理后的酿酒酵母细胞中研究了PtdEtn易位。通过使用荧光胺作为外膜探针，测量ATP耗尽、ATP耗尽后再补充以及N-乙基马来酰亚胺处理的细胞中的外部PtdEtn水平，来研究PtdEtn易位。分别用各种细胞松弛素和秋水仙碱（或苯菌灵）处理破坏微丝和微管。在细胞松弛素处理的细胞中，PtdEtn易位变得异常，但在用微管破坏剂如秋水仙碱或苯菌灵处理的细胞中则没有。这些结果被解释为表明肌动蛋白细胞骨架参与调节酵母细胞质膜中的PtdEtn转位酶活性。
