Wen Z Y, Song L C, Yan Z Y, Lu Z G, Sun D, Chien S
Department of Medical Physics, Beijing Medical University, P. R. China.
Clin Hemorheol Microcirc. 1998 Apr;18(1):75-84.
Using the method of inducing spherocytic anemia in the rabbit with antibody serum, we have developed an animal model in which red blood cells (RBCs) can nearly grow synchronously. With this model, we determined that the surface charge density on the RBC membrane decreased with cell aging. The change was not linear, being much more profound in the latter half of RBC life span. There was a positive correlation between the mean RBC density and its "age" (r = 0.847, p < 0.01). However, the density distribution of the RBCs at the same "age" showed a broad range, and the density values for RBC groups with different ages showed considerable overlap. This indicates that the density gradient technique can be used to separate RBC population into fractions with different mean ages, but has a low resolving power for obtaining individual RBCs of a given "age".
我们采用抗体血清诱导家兔发生球形红细胞贫血的方法，建立了一种红细胞（RBC）几乎能同步生长的动物模型。利用该模型，我们确定红细胞膜表面电荷密度随细胞衰老而降低。这种变化并非呈线性，在红细胞寿命的后半期更为显著。红细胞平均密度与其“年龄”之间存在正相关（r = 0.847，p < 0.01）。然而，相同“年龄”的红细胞密度分布范围较宽，不同年龄红细胞组的密度值有相当程度的重叠。这表明密度梯度技术可用于将红细胞群体分离为具有不同平均年龄的组分，但对于获取给定“年龄”的单个红细胞，其分辨能力较低。
