Gobbetti A, Bellini-Cardellini L, Zerani M
Department of Molecular, Cellular, and Animal Biology, University of Camerino, Italy.
Prostaglandins Other Lipid Mediat. 1998 Apr;55(5-6):277-90. doi: 10.1016/s0090-6980(98)00026-4.
The aim of this study was to clarify the possible involvement of nitric oxide (NO) on prostaglandin (PG) E2-9-ketoreductase activity in the gonadotropin-releasing hormone (GnRH)-dependent PGF2 alpha synthesis by the interrenal gland of the female water frog, Rana esculenta, during the post-reproduction. Interrenal glands were incubated in vitro with GnRH, NO donor (sodium nitroprusside, SNP), and inhibitors of phospholipase C (compound 48/80), inositol triphosphate (decavanadate), calmodulin (calmidazolium), NO synthase (L-NAME), and PGE2-9-ketoreductase (palmitic acid). Production of PGE2 and PGF2 alpha and NO synthase and PGE2-9-ketoreductase activities were determined. GnRH and SNP increased PGF2 alpha production and PGE2-9-ketoreductase activity, and decreased production of PGE2 and GnRH increased NO synthase activity. GnRH effects were blocked by all inhibitors, except for palmitic acid, which did not affect NO synthase activity, which is increased by GnRH. This study indicates that NO may be involved in regulation of the R. esculenta post-reproduction through stimulation of PGE2-9-ketoreductase activity in GnRH-dependent PGF2 alpha synthesis by the frog interrenal gland.
本研究的目的是阐明一氧化氮（NO）可能参与雌性食用蛙（Rana esculenta）产后促性腺激素释放激素（GnRH）依赖性前列腺素F2α（PGF2α）合成过程中前列腺素（PG）E2-9-酮还原酶活性的调控机制。将产后雌性食用蛙的肾间组织在体外分别与GnRH、NO供体（硝普钠，SNP）以及磷脂酶C抑制剂（化合物48/80）、肌醇三磷酸抑制剂（十钒酸盐）、钙调蛋白抑制剂（氯米帕明）、NO合酶抑制剂（L-硝基精氨酸甲酯，L-NAME）和PGE2-9-酮还原酶抑制剂（棕榈酸）共同孵育。测定PGE2和PGF2α的生成量以及NO合酶和PGE2-9-酮还原酶的活性。GnRH和SNP可增加PGF2α的生成量及PGE2-9-酮还原酶的活性，降低PGE2的生成量，且GnRH可提高NO合酶的活性。除棕榈酸外，所有抑制剂均能阻断GnRH的作用，棕榈酸不影响GnRH所增强的NO合酶活性。本研究表明，NO可能通过刺激蛙肾间组织GnRH依赖性PGF2α合成过程中的PGE2-9-酮还原酶活性，参与食用蛙产后的调节过程。
