Yatskin O N, Philippova M M, Karelin A A, Ivanov V T
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Moscow.
FEBS Lett. 1998 May 29;428(3):286-90. doi: 10.1016/s0014-5793(98)00549-3.
Screening of hemorphins in extracts of rat lung, brain, heart and spleen was carried out. The threshold for detection of hemorphins was 0.01 nmol for spleen and 0.05 nmol for other tissues. Both the content and the composition of hemorphins differed significantly in the tissues analyzed. Heart and lung extracts were rich in these peptides, the content of the most abundant components reaching 16-44 nmol/g of tissue. In contrast, spleen and brain contained much lower amounts of hemorphins, i.e. about 0.3-2.6 nmol/g of tissue. The most represented hemorphin in lung, heart and brain was VV-hemorphin-5, while the content of other members of the hemorphin family depended significantly on the tissue analyzed: lung extract was also rich in LVV-hemorphin-5, heart contained similar amounts of LVV-hemorphin-7 and LVV-hemorphin-5 and brain of LVV-hemorphin-6. In contrast, the hemorphin family in spleen was represented mainly by C-terminally shortened VV-hemorphins, i.e. VV-hemorphin-4 and VV-hemorphin-3. The levels of hemorphins in all cases were sufficient to activate the opioid receptors of the respective tissues.
对大鼠肺、脑、心脏和脾脏提取物中的血啡肽进行了筛查。脾脏血啡肽的检测阈值为0.01 nmol，其他组织为0.05 nmol。在所分析的组织中，血啡肽的含量和组成均有显著差异。心脏和肺提取物中富含这些肽，最丰富成分的含量达到16 - 44 nmol/g组织。相比之下，脾脏和大脑中的血啡肽含量要低得多，即约0.3 - 2.6 nmol/g组织。肺、心脏和大脑中最具代表性的血啡肽是VV - 血啡肽 - 5，而血啡肽家族其他成员的含量在很大程度上取决于所分析的组织：肺提取物中也富含LVV - 血啡肽 - 5，心脏中LVV - 血啡肽 - 7和LVV - 血啡肽 - 5的含量相似，大脑中则富含LVV - 血啡肽 - 6。相比之下，脾脏中的血啡肽家族主要由C端缩短的VV - 血啡肽代表，即VV - 血啡肽 - 4和VV - 血啡肽 - 3。在所有情况下，血啡肽的水平都足以激活相应组织的阿片受体。
