Dutta J, Biswas A, Saha S, Deb C
J Chromatogr. 1976 Sep 1;124(1):29-36. doi: 10.1016/s0021-9673(00)87833-4.
A method for the estimation of both free and bound cholesterol in serum and plasma involving thin-layer chromatography has been developed. The serum or plasma is applied as a band directly on the plate. Extraction of lipid material from this band and the chromatographic separation of the above components are carried out on the same plate. The components are extracted from the adsorbent directly with acetic acid and measured colorimetrically using iron(III) chloride-sulphuric acid reagent.
已开发出一种利用薄层色谱法估算血清和血浆中游离胆固醇和结合胆固醇的方法。血清或血浆直接以条带形式点样于薄板上。从该条带中提取脂质物质并在同一薄板上对上述成分进行色谱分离。直接用乙酸从吸附剂中提取各成分，并用氯化铁 - 硫酸试剂进行比色测定。
