Peter F, Reynolds R G
J Chromatogr. 1977 Mar 1;143(2):152-60.
A fast, inexpensive, simple method is described for the determination of the total cholesterol concentration in human serum. The cholesterols (free and esterified) are extracted from serum by a double extraction procedure, using isopropanol-water-10 N sodium hydroxide (250:125:10) and then n-octane. An aliquot of the octane extract is spotted on a thin-layer plate and the cholesterol in the spot is rendered visible using an aqueous phosphomolybdic acid staining solution and a heat treatment for the color development. The colored spot is scanned with a densitometer.
本文描述了一种快速、廉价、简单的方法，用于测定人血清中的总胆固醇浓度。通过双提取程序从血清中提取胆固醇（游离胆固醇和酯化胆固醇），先用异丙醇-水-10N氢氧化钠（250:125:10），然后用正辛烷。取一份正辛烷提取物点在薄层板上，用磷钼酸水溶液染色液显色，并通过热处理进行显色，使斑点中的胆固醇可见。用密度计扫描有色斑点。
