Flynn K J, Belz G T, Altman J D, Ahmed R, Woodland D L, Doherty P C
Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Immunity. 1998 Jun;8(6):683-91. doi: 10.1016/s1074-7613(00)80573-7.
Virus-specific CD8+ effector T cells (eCTL) are enriched in the lungs of mice with primary influenza pneumonia, though later detection of memory T cells (mCTL) in the mediastinal lymph nodes (MLN) or spleen by peptide-based staining protocols is at the limits of flow cytometric analysis. Respiratory challenge with an H3N2 virus months after H1N1 priming induces a massive recall response, which reduces virus titers 2-3 days earlier than in nave controls. Influenza-specific mCTL produce interferon-gamma within 6 hr, but still take 4-5 days to localize to the infected respiratory tract. The delay reflects that the recall response develops first in the MLN, which contains relatively few mCTL. The response to a subdominant epitope is less obvious after secondary challenge.
在原发性流感肺炎小鼠的肺中，病毒特异性CD8 +效应T细胞（eCTL）富集，不过随后通过基于肽的染色方案在纵隔淋巴结（MLN）或脾脏中检测记忆T细胞（mCTL）处于流式细胞术分析的极限。H1N1初次免疫数月后用H3N2病毒进行呼吸道攻击会引发大规模的回忆反应，该反应比未接触过抗原的对照提前2 - 3天降低病毒滴度。流感特异性mCTL在6小时内产生γ干扰素，但仍需4 - 5天才能定位于受感染的呼吸道。这种延迟反映出回忆反应首先在含有相对较少mCTL的MLN中发展。二次攻击后对次要表位的反应不太明显。
