Ko R C
Department of Zoology, University of Hong Kong, Hong Kong.
Southeast Asian J Trop Med Public Health. 1997;28 Suppl 1:91-8.
The latest immunological and molecular methods for the diagnosis of swine and human trichinellosis are briefly reviewed. The following topics are discussed in more detail: isolation of specific antigens by continuous elution-isoelectric focusing methods, production of recombinant antigens, nature of immunodominant antigens, potential use of heat shock proteins (HSPs) as diagnostic antigens, roles of specific IgE and circulating antigens (CA). The immunodominant antigens were found to be highly heat resistant. The specificity and sensitivity of colorimetric sandwich ELISA, microfluorescence (ELFA), enhanced chemiluminescence (ECIA) and dissociated enhanced lanthanide fluoroimmunoassay (DELFIA) in detecting CA were compared. The last method is the most sensitive, detecting as little as 1 ng of antigens/ml of serum. CA was detected as early as 7 days postinfection of mice. The serum from a patient suspected to have acute trichinellosis in Hong Kong was also tested positive for CA.
本文简要回顾了诊断猪和人旋毛虫病的最新免疫学和分子方法。更详细地讨论了以下主题：通过连续洗脱-等电聚焦方法分离特异性抗原、重组抗原的产生、免疫显性抗原的性质、热休克蛋白（HSPs）作为诊断抗原的潜在用途、特异性IgE和循环抗原（CA）的作用。发现免疫显性抗原具有高度耐热性。比较了比色夹心ELISA、微荧光（ELFA）、增强化学发光（ECIA）和解离增强镧系元素荧光免疫分析（DELFIA）检测CA的特异性和灵敏度。最后一种方法最灵敏，能检测低至1 ng抗原/毫升血清。感染小鼠后最早在7天就能检测到CA。对香港一名疑似患有急性旋毛虫病患者的血清检测，CA也呈阳性。
