嗜热栖热放线菌中个别纤维素酶生物合成的调控。
Regulation of biosynthesis of individual cellulases in Thermomonospora fusca.
作者信息
Spiridonov N A, Wilson D B
机构信息
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA.
出版信息
J Bacteriol. 1998 Jul;180(14):3529-32. doi: 10.1128/JB.180.14.3529-3532.1998.
Abstract
Regulation of the biosynthesis of the six cellulases comprising the cellulolytic system of the thermophilic soil bacterium Thermomonospora fusca ER1 was studied. The levels of the individual enzymes produced on different noninducing and inducing carbon sources were determined. The lowest level of cellulase synthesis (3 nM) was observed with xylose as a carbon source, and the highest level (247 to 1,670 nM for different enzymes) was found in cultures grown on microcrystalline cellulose. Endocellulases and exocellulases showed distinctly different regulation patterns. Differences in the regulation of individual enzymes appear to be determined by the specific structural organization of the upstream regulatory sequences of their genes.
摘要
对嗜热土壤细菌栖热单胞菌ER1纤维素分解系统中六种纤维素酶生物合成的调控进行了研究。测定了在不同非诱导和诱导碳源上产生的各个酶的水平。以木糖作为碳源时观察到纤维素酶合成的最低水平（3 nM），而在微晶纤维素上生长的培养物中发现了最高水平（不同酶为247至1670 nM）。内切纤维素酶和外切纤维素酶表现出明显不同的调控模式。各个酶调控的差异似乎由其基因上游调控序列的特定结构组织所决定。
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