Vanee Niti, Brooks J Paul, Fong Stephen S
VCU Life Sciences, Virginia Commonwealth University, Richmond 23284, VA, USA.
Chemical and Life Sciences Engineering, Virginia Commonwealth University, Richmond 23284, VA, USA.
Metabolites. 2017 Nov 11;7(4):57. doi: 10.3390/metabo7040057.
Actinomycetes have a long history of being the source of numerous valuable natural products and medicinals. To expedite product discovery and optimization of biochemical production, high-throughput technologies can now be used to screen the library of compounds present (or produced) at a given time in an organism. This not only facilitates chemical product screening, but also provides a comprehensive methodology to the study cellular metabolic networks to inform cellular engineering. Here, we present some of the first metabolomic data of the industrial cellulolytic actinomycete generated using LC-MS/MS. The underlying objective of conducting global metabolite profiling was to gain better insight on the innate capabilities of , with a long-term goal of facilitating -based bioprocesses. The metabolome was characterized for growth on two cellulose-relevant carbon sources, cellobiose and Avicel. Furthermore, the comprehensive list of measured metabolites was computationally integrated into a metabolic model of , to study metabolic shifts in the network flux associated with carbohydrate and amino acid metabolism.
放线菌作为众多有价值的天然产物和药物的来源已有很长的历史。为了加快产品发现和生物化学产品优化，现在可以使用高通量技术来筛选生物体在给定时间存在（或产生）的化合物库。这不仅有助于化学产品筛选，还为研究细胞代谢网络以指导细胞工程提供了一种全面的方法。在这里，我们展示了使用LC-MS/MS生成的工业纤维素分解放线菌的首批代谢组学数据中的一些。进行全局代谢物谱分析的基本目标是更好地了解该菌的固有能力，长期目标是促进基于该菌的生物过程。对该菌在两种与纤维素相关的碳源（纤维二糖和微晶纤维素）上生长的代谢组进行了表征。此外，将测得的代谢物综合列表通过计算整合到该菌的代谢模型中，以研究与碳水化合物和氨基酸代谢相关的网络通量中的代谢变化。
