Ingavale S S, Kaur R, Aggarwal P, Bachhawat A K
Institute of Microbial Technology, Chandigarh-160 036, India.
J Bacteriol. 1998 Jul;180(14):3727-9. doi: 10.1128/JB.180.14.3727-3729.1998.
We describe the presence of a minisatellite sequence that displays length polymorphisms in the fission yeast Schizosaccharomyces pombe. The minisatellite sequence was found to reside within the propeptide region of the vacuolar carboxypeptidase Y gene. The minisatellite sequence, which was found only at a single locus, was mitotically stable and displayed length polymorphisms between the two varieties of S. pombe (S. pombe var. pombe and S. pombe var. malidevorans). The minisatellite sequence, however, appeared to be species specific and was absent in other members of the Schizosaccharomyces genus. This report constitutes the first experimental demonstration of the presence of such sequences in yeasts.
我们描述了一种微卫星序列的存在,该序列在裂殖酵母粟酒裂殖酵母中表现出长度多态性。发现该微卫星序列位于液泡羧肽酶Y基因的前肽区域内。该微卫星序列仅在单个位点被发现,在有丝分裂过程中是稳定的,并且在粟酒裂殖酵母的两个变种(粟酒裂殖酵母变种粟酒裂殖酵母和粟酒裂殖酵母变种苹果酸分解菌)之间表现出长度多态性。然而,该微卫星序列似乎具有物种特异性,在裂殖酵母属的其他成员中不存在。本报告首次通过实验证明了酵母中存在此类序列。