Mertins P, Gallwitz D
Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG.
Nucleic Acids Res. 1987 Sep 25;15(18):7369-79. doi: 10.1093/nar/15.18.7369.
The actin gene of the fission yeast Schizosaccharomyces pombe has been isolated by using as a hybridization probe cloned actin DNA from the budding yeast Saccharomyces cerevisiae. In contrast to most actin genes studied from diverse eukaryotic species, the S. pombe gene is not interrupted by introns. The protein sequence deduced from the nucleotide sequence of the gene shows that the S. pombe actin is more closely related to the mammalian gamma-actin than to the actin of S. cerevisiae. Three transcripts of 1240, 1650 and 1850 nucleotides having the same 5' end but differing in the length of their 3' untranslated region are generated in the fission yeast. Only one messenger RNA of 1330 nucleotides is formed from the S. pombe actin gene in S. cerevisiae. Contrary to the observation made with other S. pombe genes transcribed in the budding yeast, the heterologous actin gene transcript is initiated 39 nucleotides upstream of the initiation start site used in the homologous yeast. The mRNA termination (or 3' processing) mechanism in the two ascomycetes also differs as the 3'end of the S. pombe actin gene transcript in S. cerevisiae does not coincide with either of the three 3'ends mapped in the fission yeast.
利用来自芽殖酵母酿酒酵母的克隆肌动蛋白DNA作为杂交探针，分离出了裂殖酵母粟酒裂殖酵母的肌动蛋白基因。与从不同真核生物物种研究的大多数肌动蛋白基因不同，粟酒裂殖酵母基因不被内含子打断。从该基因的核苷酸序列推导的蛋白质序列表明，粟酒裂殖酵母肌动蛋白与哺乳动物γ-肌动蛋白的关系比与酿酒酵母肌动蛋白的关系更密切。在裂殖酵母中产生了三种1240、1650和1850个核苷酸的转录本，它们具有相同的5'端，但3'非翻译区长度不同。在酿酒酵母中，粟酒裂殖酵母肌动蛋白基因只形成一种1330个核苷酸的信使RNA。与在芽殖酵母中转录的其他粟酒裂殖酵母基因的观察结果相反，异源肌动蛋白基因转录本在同源酵母中使用的起始位点上游39个核苷酸处起始。这两种子囊菌中的mRNA终止（或3'加工）机制也不同，因为酿酒酵母中粟酒裂殖酵母肌动蛋白基因转录本的3'端与裂殖酵母中定位的三个3'端都不一致。
