Yamaguchi A, Goi T, Taguchi S, Ohtaki N, Seki K, Hirose K, Nakagawara G, Urano T, Furukawa K
First Department of Surgery, School of Medicine, Fukui Medical University, Japan.
J Gastroenterol. 1998 Jun;33(3):349-53. doi: 10.1007/s005350050095.
We examined serum levels of a CD44 splice variant that contained variant exons 8-10 (CD44v8-10) as a tumor marker in colorectal cancer patients. We performed enzyme-linked immunosorbent assays in 81 sera obtained from 71 colorectal cancer patients and 10 healthy controls. Serum CD44v8-10 levels were significantly higher in the colorectal cancer patients than in the healthy controls (0.209 +/- 0.098 versus 0.114 +/- 0.019 OD; P < 0.01). There was a close correlation between immunohistochemical expression and serum CD44v8-10 levels. Surgical resection of the tumors resulted in a reduction of serum CD44v8-10 levels. There was no significant correlation between serum CD44v8-10 level and serosal invasion or histologic type. However, a significant correlation was observed between serum CD44v8-10 level and lymphatic or venous invasion. In addition, serum CD44v8-10 levels were significantly higher in carcinomas associated with lymph node or liver metastasis than in those without metastasis. These findings suggest the usefulness of serum CD44v8-10 level in the prediction of colorectal cancer metastasis.
我们检测了一种包含可变外显子8 - 10的CD44剪接变体（CD44v8 - 10）的血清水平，将其作为结直肠癌患者的肿瘤标志物。我们对71例结直肠癌患者和10例健康对照者的81份血清进行了酶联免疫吸附测定。结直肠癌患者血清CD44v8 - 10水平显著高于健康对照者（0.209±0.098对0.114±0.019 OD；P < 0.01）。免疫组化表达与血清CD44v8 - 10水平之间存在密切相关性。肿瘤手术切除导致血清CD44v8 - 10水平降低。血清CD44v8 - 10水平与浆膜侵犯或组织学类型之间无显著相关性。然而，血清CD44v8 - 10水平与淋巴或静脉侵犯之间存在显著相关性。此外，与有淋巴结或肝转移的癌相比，无转移的癌血清CD44v8 - 10水平显著更高。这些发现提示血清CD44v8 - 10水平在预测结直肠癌转移方面具有实用性。
