Yamaguchi A, Saito M, Gio T, Iida A, Takeuchi K, Hirose K, Nakagawara G, Urano T, Furukawa K, Shiku H
First Department of Surgery, Fukui Medical School.
Jpn J Cancer Res. 1995 Dec;86(12):1166-71. doi: 10.1111/j.1349-7006.1995.tb03310.x.
The expression of CD44 variant containing variant exons 8-10 product (CD44v8-10) was studied by western blot analysis and immunohistochemistry in gastric cancers using a monoclonal antibody, 44-1V. On western blots, a single band of 130 kD was recognized in stomach cancer cell lines. CD44v8-10 expression, with reactivity localized in the cell membrane, was found in 65 (33.5%) of the 194 advanced gastric cancers. There was no correlation between CD44v8-10 immunoreactivity and serosal, lymphatic, or lymph node invasion. However, there was significant correlation with CD44v8-10 immunoreactivity and venous invasion. CD44v8-10-positive cancers were more frequently associated with hematogenous metastasis than those which were immunonegative. There was an inverse association between CD44v8-10 immunoreactivity and peritoneal dissemination, especially in diffuse type adenocarcinomas. These observations indicate that CD44v8-10 may play a role in the metastasis of gastric cancer.
采用单克隆抗体44-1V，通过蛋白质印迹分析和免疫组织化学方法，研究了含可变外显子8-10产物的CD44变体（CD44v8-10）在胃癌中的表达。在蛋白质印迹上，在胃癌细胞系中识别出一条130 kD的单条带。在194例进展期胃癌中，65例（33.5%）发现CD44v8-10表达，其反应性定位于细胞膜。CD44v8-10免疫反应性与浆膜、淋巴管或淋巴结侵犯之间无相关性。然而，CD44v8-10免疫反应性与静脉侵犯之间存在显著相关性。与免疫阴性的癌症相比，CD44v8-10阳性的癌症更常发生血行转移。CD44v8-10免疫反应性与腹膜播散呈负相关，尤其是在弥漫型腺癌中。这些观察结果表明，CD44v8-10可能在胃癌转移中起作用。
