胆固醇的一种新的酶法测定。利用醛脱氢酶测定产生过氧化氢的反应，II。
A new enzymatic determination of cholesterol. The use of aldehyde dehydrogenase to measure H2O2 producing reactions, II.
作者信息
Haeckel R, Perlick M
出版信息
J Clin Chem Clin Biochem. 1976 Aug;14(8):411-4. doi: 10.1515/cclm.1976.14.1-12.411.
PMID:965895
Abstract
Cholesterol oxidase, coupled with the catalase and aldehyde dehydrogenase, is proposed for the determination of cholesterol. The main advantages of this procedure over comparable methods employing cholesterol oxidase are its short reaction time and the use of NADP which permits the direct calculation of the cholesterol concentration from the absorbance value.
摘要
胆固醇氧化酶与过氧化氢酶和醛脱氢酶联用，用于胆固醇的测定。与使用胆固醇氧化酶的类似方法相比，该方法的主要优点是反应时间短，且使用了烟酰胺腺嘌呤二核苷酸磷酸（NADP），从而可以根据吸光度值直接计算胆固醇浓度。
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