一种测定葡萄糖的新酶法。
A new enzymatic method for the determination of glucose.
作者信息
Heinz F, Beushausen T W
出版信息
J Clin Chem Clin Biochem. 1981 Sep;19(9):977-8. doi: 10.1515/cclm.1981.19.9.977.
PMID:7288378
Abstract
A method for the determination of glucose is described. H2O2, produced by the action of glucose oxidase, is measured from the change in absorbance due to oxidation of NAD(P)H in the presence of catalase, aldehyde dehydrogenase and a high concentration of ethanol. The quality data of the method are equivalent to those of the hexokinase-glucose-6-phosphate dehydrogenase method used as reference.
摘要
描述了一种测定葡萄糖的方法。葡萄糖氧化酶作用产生的H2O2，在过氧化氢酶、醛脱氢酶和高浓度乙醇存在的情况下，通过NAD(P)H氧化引起的吸光度变化来测定。该方法的质量数据与用作参考的己糖激酶-葡萄糖-6-磷酸脱氢酶法相当。
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