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一种测定葡萄糖的新酶法。

A new enzymatic method for the determination of glucose.

作者信息

Heinz F, Beushausen T W

出版信息

J Clin Chem Clin Biochem. 1981 Sep;19(9):977-8. doi: 10.1515/cclm.1981.19.9.977.

DOI:10.1515/cclm.1981.19.9.977
PMID:7288378
Abstract

A method for the determination of glucose is described. H2O2, produced by the action of glucose oxidase, is measured from the change in absorbance due to oxidation of NAD(P)H in the presence of catalase, aldehyde dehydrogenase and a high concentration of ethanol. The quality data of the method are equivalent to those of the hexokinase-glucose-6-phosphate dehydrogenase method used as reference.

摘要

描述了一种测定葡萄糖的方法。葡萄糖氧化酶作用产生的H2O2,在过氧化氢酶、醛脱氢酶和高浓度乙醇存在的情况下,通过NAD(P)H氧化引起的吸光度变化来测定。该方法的质量数据与用作参考的己糖激酶-葡萄糖-6-磷酸脱氢酶法相当。

相似文献

1
A new enzymatic method for the determination of glucose.一种测定葡萄糖的新酶法。
J Clin Chem Clin Biochem. 1981 Sep;19(9):977-8. doi: 10.1515/cclm.1981.19.9.977.
2
[Enzyme kinetic determination of glucose (author's transl)].葡萄糖的酶动力学测定(作者译)
J Clin Chem Clin Biochem. 1976 Jan;14(1):27-30.
3
[ENZYMATIC METHODS FOR THE DETERMINATION OF BLOOD AND URINE SUGAR WITH REFERENCE TO COMPARATIVE STUDIES WITH CLASSICAL METHODS].[酶法测定血糖和尿糖及与经典方法的比较研究]
Internist (Berl). 1963 Dec;4:554-9.
4
Simple automated determination of serum or plasma glucose by a hexokinase-glucose-6 -phosphate dehydrogenase method.采用己糖激酶-葡萄糖-6-磷酸脱氢酶法简单自动测定血清或血浆葡萄糖。
Clin Chem. 1972 Jun;18(6):509-15.
5
A new enzymatic determination of cholesterol. The use of aldehyde dehydrogenase to measure H2O2 producing reactions, II.胆固醇的一种新的酶法测定。利用醛脱氢酶测定产生过氧化氢的反应,II。
J Clin Chem Clin Biochem. 1976 Aug;14(8):411-4. doi: 10.1515/cclm.1976.14.1-12.411.
6
Single- and coupled-enzyme nylon-tube reactors for plasma glucose determination with glucose oxidase and aldehyde dehydrogenase.用于葡萄糖氧化酶和乙醛脱氢酶测定血浆葡萄糖的单酶和双酶尼龙管反应器。
Clin Chem. 1980 Nov;26(12):1652-5.
7
[Determination of glucose with glucoseoxidase-UV, using hexokinase as the reference method (author's transl)].
J Clin Chem Clin Biochem. 1976 Jul;14(7):373-4.
8
Enzymatic determination of D-mannose in serum.血清中 D-甘露糖的酶法测定。
Clin Chem. 1984 Feb;30(2):293-4.
9
A kinetic method for glucose that is insensitive to variations in temperature and enzyme activity.
Clin Chem. 1979 Sep;25(9):1581-90.
10
[Sources of error in enzymatic determination of blood glucose].
Monatsschr Kinderheilkd (1902). 1969 Apr;117(4):264-7.

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