利用X连锁多态性对分离的胃肠化生腺体进行克隆分析。
Clonal analysis of isolated intestinal metaplastic glands of stomach using X linked polymorphism.
作者信息
Nomura S, Kaminishi M, Sugiyama K, Oohara T, Esumi H
机构信息
Third Department of Surgery, Faculty of Medicine, University of Tokyo, Japan.
出版信息
Gut. 1998 May;42(5):663-8. doi: 10.1136/gut.42.5.663.
BACKGROUND
Monoclonal precancerous cells undergo successive biochemical and genetic changes during the multistep process of carcinogenesis in the gastrointestinal tract. Despite a high association with intestinal-type stomach cancer (differentiated adenocarcinoma of the stomach), the role of intestinal metaplasia is unclear in stomach carcinogenesis.
AIMS
To study the clonality of intestinal metaplasia.
METHODS
The clonality of 86 single intestinal metaplastic glands isolated by EDTA treatment from gastrectomy specimens from patients with cancer were investigated. The methylation sensitive restriction enzyme HpaII and polymerase chain reaction (PCR) were used to detect a polymorphic human androgen receptor gene locus linked to an inactive X chromosome.
RESULTS
Forty one (48%) intestinal metaplastic glands were heterotypic (mixed cells of different allelic methylation) and 45 (52%) were homotypic (cell population of the same allelic methylation), while almost all the single pyloric glands were homotypic. Eleven of 13 intestinal metaplastic mucosae that were 6 mm in diameter contained glands that had originated from different cells. There were no strong relationships between clonal type and location or histological type of intestinal metaplasia.
CONCLUSION
Intestinal metaplasia in general is not a lesion that arises or proceeds monoclonally.
背景
单克隆癌前细胞在胃肠道癌变的多步骤过程中经历连续的生化和基因变化。尽管肠化生与肠型胃癌（胃分化腺癌）高度相关，但肠化生在胃癌发生中的作用尚不清楚。
目的
研究肠化生的克隆性。
方法
对86个通过EDTA处理从癌症患者胃切除标本中分离出的单个肠化生腺体的克隆性进行了研究。使用甲基化敏感限制性内切酶HpaII和聚合酶链反应（PCR）检测与失活X染色体相关的多态性人类雄激素受体基因位点。
结果
41个（48%）肠化生腺体为异型（不同等位基因甲基化的混合细胞），45个（52%）为同型（相同等位基因甲基化的细胞群体），而几乎所有单个幽门腺均为同型。13个直径为6 mm的肠化生黏膜中有11个包含起源于不同细胞的腺体。克隆类型与肠化生的位置或组织学类型之间没有密切关系。
结论
一般来说，肠化生不是单克隆发生或进展的病变。
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