Shimoyama T, Yoshimura T, Mikami T, Fukuda S, Crabtree J E, Munakata A
First Department of Internal Medicine, Hirosaki University School of Medicine, Japan.
J Clin Pathol. 1998 Apr;51(4):299-301. doi: 10.1136/jcp.51.4.299.
To examine the vacA genotypes of Helicobacter pylori strains in Japan and to define whether any specific genotype was associated with gastric cancer.
The allelic variation of vacA gene was studied using a recently introduced polymerase chain reaction based vacA genotyping system.
80 H pylori strains were isolated from gastric biopsies of 40 patients with gastric cancer and 40 control subjects in a Japanese population. All strains were s1/m1 subtype and 79 of 80 strains were classified as s1a subtype.
The recently proposed vacA genotyping system is applicable to Japanese H pylori strains and most strains have the s1a genotype, associated with increased virulence. While the high frequency of s1a/m1 vacA genotype might play a role in the increased incidence of atrophic gastritis and gastric cancer in Japanese subjects, it precludes its use as a predictor of clinical outcome of H pylori infection in Japan.
检测日本幽门螺杆菌菌株的vacA基因型，并确定是否有任何特定基因型与胃癌相关。
使用最近引入的基于聚合酶链反应的vacA基因分型系统研究vacA基因的等位基因变异。
从日本人群中40例胃癌患者和40例对照受试者的胃活检组织中分离出80株幽门螺杆菌菌株。所有菌株均为s1/m1亚型，80株中有79株被分类为s1a亚型。
最近提出的vacA基因分型系统适用于日本幽门螺杆菌菌株，大多数菌株具有s1a基因型，与毒力增加相关。虽然s1a/m1 vacA基因型的高频率可能在日本受试者萎缩性胃炎和胃癌发病率增加中起作用，但它不能作为日本幽门螺杆菌感染临床结局的预测指标。
