Mooney M A, Vaughn D M, Reinhart G A, Powers R D, Wright J C, Hoffman C E, Swaim S F, Baker H J
Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, AL 36849-5525, USA.
Am J Vet Res. 1998 Jul;59(7):859-63.
To ascertain the effects of dietary omega-3 (n-3) fatty acids on biochemical and histopathologic components of the inflammatory stage of wound healing.
30 purpose-bred Beagles.
Dogs were allotted to 5 groups of 6. Each group was fed a unique dietary fatty acid ratio of omega-6 to n-3--diet A, 5.3:1; diet B, 10.4:1; diet C, 24.1:1; diet D, 51.6:1; and diet E, 95.8:1. Dogs were fed once daily for 12 weeks, then biopsy specimens were taken from 4-day-old wounds of each dog and analyzed by gas chromatography-mass spectrometry for: prostaglandin E2 (PGE2) metabolites, and ratios of omega-6 to n-3 fatty acids, arachidonic acid (AA) to eicosapentaenoic acid (EPA), adrenic acid to docosahexaenoic acid, and PGE2 to prostaglandin E3 (PGE3) metabolites.
Qualitative analysis was carried out on AA, EPA, adrenic acid, docosahexaenoic acid, and the major metabolite from the PGE2 and PGE3 pathway. These molecules were further quantified with respect to diet to determine significant differences. By analysis of the AA-to-EPA ratio, diet A was different from diets D and E and diets B and C were different from diet E (P < 0.05). By analysis of the PGE2-to-PGE3 metabolite ratio, diet A was different from diet E (P < 0.05). Though biochemical analysis indicated dietary dependence, histopathologic data indicated no significant difference with respect to diet groups.
The biochemical component of the inflammatory stage of wound healing can be manipulated by diet.
Omega-3 fatty acid-enriched diets can be used to control inflammation associated with dermatologic conditions.
确定膳食ω-3（n-3）脂肪酸对伤口愈合炎症期生化和组织病理学成分的影响。
30只专门培育的比格犬。
将犬分为5组，每组6只。每组喂食具有独特ω-6与n-3膳食脂肪酸比例的食物——饮食A，5.3:1；饮食B，10.4:1；饮食C，24.1:1；饮食D，51.6:1；饮食E，95.8:1。犬每天喂食一次，持续12周，然后从每只犬4日龄的伤口处采集活检标本，通过气相色谱-质谱联用仪分析：前列腺素E2（PGE2）代谢物，以及ω-6与n-3脂肪酸、花生四烯酸（AA）与二十碳五烯酸（EPA）、肾上腺酸与二十二碳六烯酸的比例，和PGE2与前列腺素E3（PGE3）代谢物的比例。
对AA、EPA、肾上腺酸、二十二碳六烯酸以及PGE2和PGE3途径的主要代谢物进行了定性分析。对这些分子进一步按饮食进行定量分析以确定显著差异。通过分析AA与EPA的比例，饮食A与饮食D和E不同，饮食B和C与饮食E不同（P<0.05）。通过分析PGE2与PGE3代谢物的比例，饮食A与饮食E不同（P<0.05）。虽然生化分析表明存在饮食依赖性，但组织病理学数据表明各饮食组之间无显著差异。
伤口愈合炎症期的生化成分可通过饮食进行调控。
富含ω-3脂肪酸的饮食可用于控制与皮肤病相关的炎症。
