Weik M, Patzelt H, Zaccai G, Oesterhelt D
Institut de Biologie Structurale, Grenbole, France.
Mol Cell. 1998 Feb;1(3):411-9. doi: 10.1016/s1097-2765(00)80041-6.
Evidence is accumulating for the lateral organization of cell membrane lipids and proteins in the context of sorting or intracellular signaling. So far, however, information has been lacking on the details of protein-lipid interactions in such aggregates. Purple membranes are patches made up of lipids and the protein bacteriorhodopsin in the plasma membrane of certain Archaea. Naturally crystalline, they provide a unique opportunity to study the structure of a natural membrane at submolecular resolution by diffraction methods. We present a direct structural determination of the glycolipids with respect to bacteriorhodopsin in these membranes. Deuterium labels incorporated in vivo into the sugar moieties of the major glycolipid were localized by neutron diffraction. The data suggest a role for specific aromatic residue-carbohydrate stacking interactions in the formation of the purple membrane crystalline patches.
在分选或细胞内信号传导的背景下，细胞膜脂质和蛋白质侧向组织的证据越来越多。然而，到目前为止，关于此类聚集体中蛋白质 - 脂质相互作用的细节信息还很缺乏。紫色膜是由某些古细菌质膜中的脂质和蛋白质细菌视紫红质组成的斑块。它们自然结晶，为通过衍射方法在亚分子分辨率下研究天然膜的结构提供了独特的机会。我们对这些膜中与细菌视紫红质相关的糖脂进行了直接的结构测定。通过中子衍射确定了体内掺入主要糖脂糖部分的氘标记的位置。数据表明特定的芳香族残基 - 碳水化合物堆积相互作用在紫色膜结晶斑块形成中起作用。
