Castignolles N, Petit F, Mendel I, Simon L, Cattolico L, Buffet-Janvresse C
Laboratorie de Virologie, Centre Hospitalier Universitaire Charles Nicolle, Rouen, France.
Mol Cell Probes. 1998 Jun;12(3):175-80. doi: 10.1006/mcpr.1998.0166.
Several systems for isolating viruses from environmental samples have been tested. The most promising method is based on genomic amplification. The authors attempted to detect adenovirus in nucleic-acid extracts from the Seine River estuary by a two-step amplification of a 220-bp segment of the conserved coding region of type 2 adenovirus hexon protein L3. The primers used in this study detected the most prevalent adenovirus serotypes in human disease in France, but not other virus strains or bacteria. The sensitivity of the nested polymerase chain reaction (PCR) amplification was estimated to be 10(2) copies of the adenovirus target sequence per ml of Seine River water. Nucleic-acid extracts from Seine River estuary waters were analysed and some tested positive for the presence of adenoviruses.
已经对几种从环境样本中分离病毒的系统进行了测试。最有前景的方法是基于基因组扩增。作者试图通过对2型腺病毒六邻体蛋白L3保守编码区的一个220碱基对片段进行两步扩增，来检测塞纳河河口核酸提取物中的腺病毒。本研究中使用的引物检测出了法国人类疾病中最常见的腺病毒血清型，但未检测出其他病毒株或细菌。巢式聚合酶链反应（PCR）扩增的灵敏度估计为每毫升塞纳河水中有10(2)个腺病毒靶序列拷贝。对塞纳河河口水域的核酸提取物进行了分析，一些样本检测出腺病毒呈阳性。
