Mulichak A M, Wilson J E, Padmanabhan K, Garavito R M
Department of Biochemistry, Michigan State University, East Lansing, USA.
Nat Struct Biol. 1998 Jul;5(7):555-60. doi: 10.1038/811.
We have determined the structures of the glucose-6-phosphate (G6P)-inhibitable 100,000 Mr Type I hexokinase from rat and the G6P-sensitive 50,000 Mr hexokinase from Schistosoma mansoni at a resolution of 2.8 and 2.6 A respectively. The structures define the glucose and G6P binding sites in these enzymes, suggest the mechanisms of intradomain G6P inhibition and activity loss in the Type I hexokinase N-terminal half, and reveal the structure of the membrane targeting motif that integrates the Type I hexokinase into the outer mitochondrial membrane.
我们分别以2.8埃和2.6埃的分辨率测定了来自大鼠的葡萄糖-6-磷酸（G6P）可抑制的100,000道尔顿I型己糖激酶以及来自曼氏血吸虫的G6P敏感的50,000道尔顿己糖激酶的结构。这些结构确定了这些酶中的葡萄糖和G6P结合位点，提示了I型己糖激酶N端结构域内G6P抑制和活性丧失的机制，并揭示了将I型己糖激酶整合到线粒体外膜中的膜靶向基序的结构。
