Vachiéry N, Trap I, Totté P, Martinez D, Bensaid A
CIRAD-EMVT, BP 515, 97165 Point à Pitre, Guadeloupe.
Ann N Y Acad Sci. 1998 Jun 29;849:181-7. doi: 10.1111/j.1749-6632.1998.tb11047.x.
Bovine endothelial cells constitutively express MHC class I molecules, whereas MHC class II molecules can be induced by interferon gamma (IFN gamma) treatment. Endothelial cells may play a role as antigen presenting cells (APC), but their role in the immune response against Cowdria ruminantium is unknown. We have studied the effect of C. ruminantium infection on the expression of MHC class I and class II molecules on the surface of bovine endothelial cells. Bovine brain endothelial cells (BBEC) from the microvasculature and bovine umbilical endothelial cells (BUEC) from the macrovasculature were cultured in vitro, infected with different concentration of Cowdria, and treated or not with IFN gamma. We observed that Cowdria induced a reduction of MHC molecules expression on the surface of BUEC and BBEC. This inhibitory effect of Cowdria on MHC molecules could affect the capacity of endothelial cells to act as efficient antigen-presenting cells.
牛内皮细胞组成性表达MHC I类分子，而MHC II类分子可通过γ干扰素（IFNγ）处理诱导表达。内皮细胞可能作为抗原呈递细胞（APC）发挥作用，但其在抵抗反刍动物考德里氏体免疫反应中的作用尚不清楚。我们研究了反刍动物考德里氏体感染对牛内皮细胞表面MHC I类和II类分子表达的影响。来自微血管的牛脑内皮细胞（BBEC）和来自大血管的牛脐内皮细胞（BUEC）在体外培养，用不同浓度的考德里氏体感染，并进行或不进行IFNγ处理。我们观察到考德里氏体诱导BUEC和BBEC表面MHC分子表达降低。考德里氏体对MHC分子的这种抑制作用可能会影响内皮细胞作为高效抗原呈递细胞的能力。
