Mendoza L, Mondragón M, Lagúnez-Otero J
Instituto de Química, UNAM, Ciudad Universitaria, Coyoacán, D.F., Mexico.
Biosystems. 1998;46(3):293-8. doi: 10.1016/s0303-2647(98)00009-4.
We evaluated the energetic stability of all possible 10-base fragments of the small subunit (SS) rRNA with a large ensemble of mRNAs. Results show that the 530 loop is the only conserved region that interacts favorably around the start codon as well as the elongation region of messengers. The interaction profile of the 530 loop around the stop codon showed that base pairing was energetically unfavorable in that region. With these results we constructed a simple scheme for the role of rRNA-mRNA interactions in the protein translation mechanism.
我们用大量的信使核糖核酸（mRNA）评估了小亚基（SS）核糖体核糖核酸（rRNA）所有可能的10碱基片段的能量稳定性。结果表明，530环是唯一在起始密码子以及信使核糖核酸的延伸区域周围具有良好相互作用的保守区域。530环在终止密码子周围的相互作用图谱表明，该区域的碱基配对在能量上是不利的。基于这些结果，我们构建了一个关于核糖体核糖核酸-信使核糖核酸相互作用在蛋白质翻译机制中作用的简单方案。
