Shichijo K, Saito H
Department of Pediatrics, Jikei University School of Medicine, Tokyo, Japan.
Int J Immunopharmacol. 1997 Nov-Dec;19(11-12):677-82. doi: 10.1016/s0192-0561(97)00097-0.
We examined the effect of anti-allergic Chinese herbal medicines such as Ma-Xing-Gan-Shi-Tang (MXGST) and Xiao-Feng-San (XFS), and a mast cell stabilizer, disodium cromoglycate (DSCG) on histamine release from mouse cultured mast cells. The mast cells (ILMCMC) were obtained by culturing mouse bone marrow cells for 3-6 weeks in the presence of IL-3. Some of the cells (FMCMC) were further cultured with a fibroblast cell line, 3T3 for 3 weeks. FMCMC had safranin-positive granules and released histamine in response to compound 48/80, whereas ILMCMC failed to do so. MXGST and XFS at 4-40 micrograms/ml inhibited IgE-dependent histamine release from ILMCMC but not from FMCMC. On the contrary, DSCG at 10(-4) M inhibited histamine release from FMCMC but not from ILMCMC. Chinese herbal medicines and DSCG may act on different types of mast cells.
我们研究了抗过敏中药如麻杏甘石汤（MXGST）和消风散（XFS）以及肥大细胞稳定剂色甘酸钠（DSCG）对小鼠培养肥大细胞组胺释放的影响。肥大细胞（ILMCMC）是通过在白细胞介素-3存在的情况下将小鼠骨髓细胞培养3至6周获得的。其中一些细胞（FMCMC）再与成纤维细胞系3T3进一步培养3周。FMCMC有番红阳性颗粒，并对化合物48/80作出反应释放组胺，而ILMCMC则不然。4至40微克/毫升的MXGST和XFS抑制ILMCMC中IgE依赖性组胺释放，但不抑制FMCMC中的释放。相反，10（-4）M的DSCG抑制FMCMC中的组胺释放，但不抑制ILMCMC中的释放。中药和DSCG可能作用于不同类型的肥大细胞。
