通过随机扩增多态性DNA（RAPD）和Southern杂交分析对小孢子菌属进行鉴别

Differentiation of Microsporum species by random amplification of polymorphic DNA (RAPD) and southern hybridization analyses.

作者信息

Kano R, Nakamura Y, Watanabe S, Takahashi H, Tsujimoto H, Hasegawa A

机构信息

Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.

出版信息

Mycoses. 1998 May-Jun;41(5-6):229-33. doi: 10.1111/j.1439-0507.1998.tb00329.x.

DOI:10.1111/j.1439-0507.1998.tb00329.x

PMID:9715638

Abstract

Molecular characterization of 18 species belonging to the genus Microsporum isolated from human and animal dermatophytosis was carried out by random amplification of polymorphic DNA (RAPD) and Southern hybridization analyses. All of these 18 Microsporum species showed different band patterns specific to each species in RAPD analysis. Southern hybridization analysis using C3 probe derived from a RAPD band from Arthroderma otae, teleomorph of M. canis, revealed distinct bands in 14 of the 18 Microsporum species but not in the other four species. The sizes of the hybridized bands of the 14 species were specific to each species, except three species (A. otae, M. equinum and M. ferrugineum) showing bands of the same size. These findings indicate that the RAPD and the Southern hybridization analyses could be effective to differentiate the species of Microsporum.

摘要

通过随机扩增多态性DNA（RAPD）和Southern杂交分析，对从人和动物皮肤癣菌病中分离出的18种小孢子菌属物种进行了分子特征分析。在RAPD分析中，所有这18种小孢子菌物种均显示出各物种特有的不同条带模式。使用源自犬小孢子菌的有性型奥塔节皮菌的RAPD条带的C3探针进行的Southern杂交分析显示，18种小孢子菌中有14种出现明显条带，而其他4种则没有。除3种（奥塔节皮菌、马小孢子菌和铁锈色小孢子菌）显示相同大小的条带外，这14种杂交条带的大小对每个物种都是特异的。这些发现表明，RAPD和Southern杂交分析可有效区分小孢子菌的物种。