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莱茵衣藻突变体多聚核糖体在体内的温度敏感性分解

Temperature-sensitive breakdown in vivo of polysomes with mutants of Chlamydomonas reinhardii.

作者信息

Cross J, McMahon D

出版信息

Mol Gen Genet. 1976 Aug 19;147(2):169-77. doi: 10.1007/BF00267568.

DOI:10.1007/BF00267568
PMID:967156
Abstract

Two temperature-sensitive mutants of Chlamydomonas reinhardii Dangeard which are defective in protein synthesis were examined. Both show breakdown of their polysomes at the restrictive temperature into monosomes which do not contain fragments of mRNA. Many of the ribosomes still contain nascent peptides able to react with puromycin. The polysome breakdown involves only cytoplasmic (80S) ribosomes and is prevented or reversed when ribosome translocation is inhibited with cyloheximide.

摘要

对莱茵衣藻丹吉尔的两个在蛋白质合成方面有缺陷的温度敏感突变体进行了研究。在限制温度下,两者的多核糖体均分解为不含mRNA片段的单核糖体。许多核糖体仍含有能与嘌呤霉素反应的新生肽。多核糖体的分解仅涉及细胞质(80S)核糖体,当用放线菌酮抑制核糖体易位时,这种分解会被阻止或逆转。

相似文献

1
Temperature-sensitive breakdown in vivo of polysomes with mutants of Chlamydomonas reinhardii.莱茵衣藻突变体多聚核糖体在体内的温度敏感性分解
Mol Gen Genet. 1976 Aug 19;147(2):169-77. doi: 10.1007/BF00267568.
2
Chloral hydrate causes breakdown of polysomes in Chlamydomonas reinhardi in vivo.水合氯醛在体内导致莱茵衣藻中的多核糖体解体。
J Biol Chem. 1976 May 10;251(9):2637-43.
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Protein synthesis in Chlamydomonas reinhardi. Evidence for synthesis of proteins of chloroplastic ribosomes on cytoplasmic ribosomes.莱茵衣藻中的蛋白质合成。关于细胞质核糖体上合成叶绿体核糖体蛋白质的证据。
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Yeast mutant, rna 1, affects the entry into polysomes of ribosomal RNA as well as messenger RNA.酵母突变体rna 1会影响核糖体RNA以及信使RNA进入多核糖体的过程。
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本文引用的文献

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A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues.一种测定动物组织中脱氧核糖核酸、核糖核酸和磷蛋白的方法。
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A simplified procedure for the preparation of tyrosine and valine-acceptor fractions of yeast "soluble ribonucleic acid".一种制备酵母“可溶性核糖核酸”的酪氨酸和缬氨酸受体组分的简化程序。
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Chloral hydrate inhibits protein synthesis in vivo.水合氯醛在体内抑制蛋白质合成。
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Role of isoleucyl-transfer ribonucleic acid synthetase in ribonucleic acid synthesis and enzyme repression in yeast.异亮氨酰 - 转移核糖核酸合成酶在酵母核糖核酸合成及酶阻遏中的作用
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