[Relation between fatty acid composition, vitamin e and malondialdehyde levels, and activity of antioxidant enzymes in the blood].

The aim of our study was to determine the composition of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) in erythrocytes and to investigate their relation to the concentration of vitamin E (Vit E) in the plasma, level of malondialdehyde (MDA) in the serum and activities of antioxidant enzymes (AE) in erythrocytes. We examined 128 healthy volunteers (57 men and 71 women) at the ages ranging between 20 and 72. Fatty acids were assessed by gas chromatography, vitamin E by the HPLC method, MDA by the spectrophotometric method, GSH-Px by the kinetic method, CU, Zn-SOD by the Randox test. We have detected the following order in the proportion of fatty acids: SFA > PUFA > MUFA. The increase in PUFA and MUFA was in direct correlation with the concentration of MDA and AE activity, however in reverse correlation with Vit E. A reverse correlation was observed in the increase in SFA. We have found an increase in lipoperoxidation due to the increase in PUFA in erythrocytes in the group of healthy people. This state is concommited by an increase in the activity of antioxidant protection for the maintenance of the balance state. The authors assume that a decrease in Vit E can be caused by its increased "consumption" as the most effective antioxidant in lipid environment. (Tab. 2, Fig. 1, Ref. 27.)