Finn T M, Amsbaugh D F
Laboratory of Pertussis, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA.
Infect Immun. 1998 Aug;66(8):3985-9. doi: 10.1128/IAI.66.8.3985-3989.1998.
Bordetella pertussis expresses a bvg-regulated 95-kDa protein, Vag8, encoded by vag-8. Southern blot analysis indicates that strains of Bordetella bronchiseptica and Bordetella parapertussis have DNA homologous to vag-8. Antiserum raised to a fusion of maltose binding protein to an N-terminal 60-kDa fragment of Vag8 recognizes the native 95-kDa protein in immunoblots of B. pertussis and B. bronchiseptica but not B. parapertussis. A 95-kDa protein-negative derivative of B. pertussis 18323 containing a deletion of vag-8 colonized mice as efficiently as the parent B. pertussis strain in a mouse aerosol model of pertussis.
百日咳博德特氏菌表达一种由vag-8编码的bvg调控的95 kDa蛋白Vag8。Southern印迹分析表明，支气管败血博德特氏菌和副百日咳博德特氏菌菌株具有与vag-8同源的DNA。用麦芽糖结合蛋白与Vag8的N端60 kDa片段的融合体产生的抗血清，在百日咳博德特氏菌和支气管败血博德特氏菌的免疫印迹中可识别天然的95 kDa蛋白，但在副百日咳博德特氏菌中则不能。在百日咳小鼠气溶胶模型中，百日咳博德特氏菌18323的一个缺失vag-8的95 kDa蛋白阴性衍生物在定殖小鼠方面与亲本百日咳博德特氏菌菌株一样有效。
