日本分离的牛A组轮状病毒G血清型和P基因型的分布
Distribution of G serotypes and P genotypes of bovine group A rotavirus isolated in Japan.
作者信息
Fukai K, Sakai T, Kamata H
机构信息
Department of Preventive Veterinary, Medicine and Animal Health, Nihon University School of Veterinary Medicine, Kanagawa, Japan.
出版信息
Aust Vet J. 1998 Jun;76(6):418-22. doi: 10.1111/j.1751-0813.1998.tb12393.x.
OBJECTIVE
To identify the distribution of G serotype and P genotype of bovine group A rotavirus in Japan.
DESIGN
Detection, isolation, electropherotyping, G serotyping and P genotyping of bovine group A rotavirus in 167 diarrhoeal faecal samples.
PROCEDURE
Bovine group A rotavirus was detected and isolated, and bovine group A rotavirus isolates were identified electropherotype by polyacrylamide gel electrophoresis and G serotype and P genotype by reverse transcription-poly-merase chain reaction.
RESULTS
Twenty-eight bovine group A rotavirus strains were isolated from 167 samples. Electropherotypes of the bovine group A rotavirus isolates were identified as long-genome electropherotype and the most prevalent combination of G serotype and P genotype of the bovine group A rotavirus isolates was G6P5 (25/28; 89.3%), followed by G6P11 (2/28; 7.1%) and G10P11 (1/28; 3.6%).
CONCLUSION
These results suggest that the bovine group A rotavirus exhibiting G6P5 is the most common in Japan.
目的
确定日本A组牛轮状病毒的G血清型和P基因型分布情况。
设计
对167份腹泻粪便样本中的A组牛轮状病毒进行检测、分离、电泳分型、G血清型鉴定和P基因分型。
方法
检测并分离A组牛轮状病毒，通过聚丙烯酰胺凝胶电泳鉴定A组牛轮状病毒分离株的电泳型，通过逆转录-聚合酶链反应鉴定G血清型和P基因型。
结果
从167份样本中分离出28株A组牛轮状病毒。A组牛轮状病毒分离株的电泳型被鉴定为长基因组电泳型，A组牛轮状病毒分离株最常见的G血清型和P基因型组合是G6P5（25/28；89.3%），其次是G-6P11（2/28；7.1%）和G10P11（1/28；3.6%）。
结论
这些结果表明，呈现G6P5的A组牛轮状病毒在日本最为常见。
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