Bottiroli G, Croce A C, Locatelli D, Nano R, Giombelli E, Messina A, Benericetti E
Centre for Histochemistry, CNR, Department of Animal Biology, University of Pavia, Italy.
Cancer Detect Prev. 1998;22(4):330-9. doi: 10.1046/j.1525-1500.1998.cdoa34.x.
The intrinsic autofluorescence properties of biological tissues can change depending on alterations induced by pathological processes. Evidence has been reported concerning the application of autofluorescence as a parameter for in situ cancer detection in several organs. In this paper, autofluorescence properties of normal and tumor tissue in the brain are described, suitable for a real-time diagnostic application. Data were obtained both on ex vivo resected samples, by microspectrofluorometric techniques, and in vivo, during surgical operation, by means of fiberoptic probe. Significant differences were found in autofluorescence emission properties between normal and tumor tissues, in terms of both spectral shape and signal amplitude, that confirm the potential of autofluorescence as a parameter to distinguish neoplastic from normal condition. The noninvasiveness of the technique opens up interesting prospects for improving the efficacy of neurosurgical operations, by allowing an intraoperative delineation of tumor resection margins.
生物组织的固有自发荧光特性会因病理过程引起的变化而改变。已有证据报道了自发荧光作为多个器官原位癌症检测参数的应用。本文描述了适用于实时诊断应用的大脑正常组织和肿瘤组织的自发荧光特性。通过显微光谱荧光技术在离体切除样本上以及在手术过程中通过光纤探头在体内获取了数据。在正常组织和肿瘤组织的自发荧光发射特性方面，在光谱形状和信号幅度上均发现了显著差异，这证实了自发荧光作为区分肿瘤与正常状态参数的潜力。该技术的非侵入性为提高神经外科手术的疗效开辟了有趣的前景，通过在术中勾勒肿瘤切除边缘来实现。
